ELISA Kit for Immunoglobulin M (IgM)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

160-100,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 59ng/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Immunoglobulin M (IgM) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Immunoglobulin M (IgM) and unlabeled Immunoglobulin M (IgM) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin M (IgM). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin M (IgM) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin M (IgM) in the sample.

Alternative Names

IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region

Item Name

Immunoglobulin M

Research Area

Infection immunity;Immune molecule;Hematology;

Reference