ELISA Kit for Immunoglobulin M (IgM)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

246.9-20000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 97.3ng/mL

Sample type

serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Immunoglobulin M (IgM) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Immunoglobulin M (IgM) and unlabeled Immunoglobulin M (IgM) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin M (IgM). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin M (IgM) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin M (IgM) in the sample.

Alternative Names

IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region

Item Name

Immunoglobulin M

Research Area

Infection immunity;Immune molecule;Hematology;

Reference