ELISA Kit for Immunoglobulin E (IgE)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

15.6-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Oryctolagus cuniculus (Rabbit)

Sensitivity

The minimum detectable dose of this kit is typically less than 7.3pg/mL

Sample type

Serum, plasma, and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin E (IgE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin E (IgE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin E (IgE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin E (IgE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region

Item Name

Immunoglobulin E

Research Area

Infection immunity;Immune molecule;Hematology;Autoimmunity;

Reference