ELISA Kit for Immunoglobulin A (IgA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78-5000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Chicken (Gallus)

Sensitivity

The minimum detectable dose of this kit is typically less than 32pg/mL

Sample type

serum, plasma and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin A (IgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin A (IgA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin A (IgA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin A (IgA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

Item Name

Immunoglobulin A

Research Area

Infection immunity;Immune molecule;Hematology;Pulmonology;

Reference

Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers;Recombinant Lactococcus lactis co-expressing OmpH of an M cell-targeting ligand and IBDV-VP2 protein provide immunological protection in chickens;Effects of and on growth performance, immune function and volatile fatty acid level of caecal digesta in broilers;