ELISA Kit for Hyaluronic Acid (HA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

4.94-400ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.71ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hyaluronic Acid (HA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hyaluronic Acid (HA) and unlabeled Hyaluronic Acid (HA) (Standards or samples) with the pre-coated antibody specific to Hyaluronic Acid (HA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hyaluronic Acid (HA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hyaluronic Acid (HA) in the sample.

Alternative Names

Hyaluronan; Hyaluronate

Item Name

Hyaluronic Acid

Research Area

Tumor immunity;Hepatology;

Reference

Hemorrhagic Fever Caused by a Novel Bunyavirus in China: Pathogenesis and Correlates of Fatal Outcome;Alveolar fluid in acute respiratory distress syndrome promotes fibroblast migration: Role of platelet-derived growth factor pathway*;Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats;Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study;Resveratrol inhibits ovarian cancer cell adhesion to peritoneal mesothelium in vitro by modulating the production of α5β1 integrins and hyaluronic acid;Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria;Pituitary tumor transforming gene as a novel regulatory factor of liver fibrosis;Camel Milk: Potential Utility as an Adjunctive Therapy to Peg-IFN/RBV in HCV-5 Infected Patients in Egypt;Study of Some Serum Biochemical Markers of Liver Fibrosis in Patients with Chronic HCV;A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritis;Polycomb group proteins: Novel molecules associated with ultraviolet A-induced photoaging of human skin.;The effect of aerobic walking and lower body resistance exercise on serum COMP and hyaluronan, in both males and females;Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis;Selected elements of extracellular matrix of the skin in diabetes and insulin resistance;