ELISA Kit for Homocysteine (HCy)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

98.77-8,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 40.22ng/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Homocysteine (HCy) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Homocysteine (HCy) and unlabeled Homocysteine (HCy) (Standards or samples) with the pre-coated antibody specific to Homocysteine (HCy). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Homocysteine (HCy) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Homocysteine (HCy) in the sample.

Alternative Names

Item Name

Homocysteine

Research Area

Reference

Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring;Homocysteinemia in relation to anemia in hypothyroid patients;