ELISA Kit for Hepcidin (Hepc)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

62.5-4,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 25.2pg/mL

Sample type

serum, plasma, urine and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepcidin (Hepc). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepcidin (Hepc). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepcidin (Hepc), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepcidin (Hepc) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor

Item Name

Hepcidin

Research Area

Metabolic pathway;Endocrinology;Hematology;Genetic science;

Reference

The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice;In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution.;Effects of iron supplementation in mice with hypoferremia induced by obesity;S-Propargyl-Cysteine, a Novel Hydrogen Sulfide Donor, Inhibits Inflammatory Hepcidin and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway.;Hepcidin links gluco-toxicity to pancreatic beta cell dysfunction by inhibiting Pdx-1 expression;inhibits ferroportin translation by modulating FBXL5‐IRP2 axis for its growth within host macrophages;