ELISA Kit for Hepatitis B Virus (HBV) Surface Antigen

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than

Sample type

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hepatitis B Virus (HBV) Surface Antigen has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hepatitis B Virus (HBV) Surface Antigen and unlabeled Hepatitis B Virus (HBV) Surface Antigen (Standards or samples) with the pre-coated antibody specific to Hepatitis B Virus (HBV) Surface Antigen. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hepatitis B Virus (HBV) Surface Antigen in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hepatitis B Virus (HBV) Surface Antigen in the sample.

Alternative Names

Item Name

Hepatitis B Virus

Research Area

Reference