ELISA Kit for Hemojuvelin (HJV)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.93ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hemojuvelin (HJV) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hemojuvelin (HJV) and unlabeled Hemojuvelin (HJV) (Standards or samples) with the pre-coated antibody specific to Hemojuvelin (HJV). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hemojuvelin (HJV) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hemojuvelin (HJV) in the sample.

Alternative Names

HFE2; HFE2A; HJV; JH; RGMC; hemochromatosis type 2(Juvenile); Haemojuvelin; RGM domain family member C

Item Name

Hemojuvelin

Research Area

Metabolic pathway;Infection immunity;Hematology;Nutrition metabolism;Hormone metabolism;

Reference

Soluble hemojuvelin in transfused and untransfused thalassaemic subjects;Urinary biomarkers predict advanced acute kidney injury after cardiovascular surgery;Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac Surgery;The functional role of hemojuvelin in acute ischemic stroke;Oxidative damage and iron dyshomeostasis in thalassemic patients;