ELISA Kit for Hemoglobin (HB)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1000ug/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Bos taurus; Bovine (Cattle)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.57ug/mL

Sample type

Serum, plasma, erythrocyte lysates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hemoglobin (HB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hemoglobin (HB) and unlabeled Hemoglobin (HB) (Standards or samples) with the pre-coated antibody specific to Hemoglobin (HB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hemoglobin (HB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hemoglobin (HB) in the sample.

Alternative Names

Hgb; Haemoglobin; Heterotetramer(αβ)2

Item Name

Hemoglobin

Research Area

Metabolic pathway;Infection immunity;Hematology;Neuro science;

Reference

Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis;