ELISA Kit for Heat Shock Protein 60 (Hsp60)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

1.56-100ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Oryctolagus cuniculus (Rabbit)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.62ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 60 (Hsp60). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 60 (Hsp60). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 60 (Hsp60), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 60 (Hsp60) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

Item Name

Heat Shock Protein 60

Research Area

Signal transduction;Tumor immunity;

Reference