ELISA Kit for Heat Shock Protein 47 (HSP47)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

125-8,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 49pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 47 (HSP47). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 47 (HSP47). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 47 (HSP47), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 47 (HSP47) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CBP2; AsTP3; CBP1; PIG14; RA-A47; SERPINH1; SERPINH2; Colligen; Gp46; Serpin Peptidase Inhibitor Clade H Member 1; Heat Shock Protein 47; Collagen Binding Protein 2

Item Name

Heat Shock Protein 47

Research Area

Developmental science;

Reference