ELISA Kit for Gonadotropin Releasing Hormone (GnRH)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Danio rerio (Zebrafish)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.39pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gonadotropin Releasing Hormone (GnRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gonadotropin Releasing Hormone (GnRH) and unlabeled Gonadotropin Releasing Hormone (GnRH) (Standards or samples) with the pre-coated antibody specific to Gonadotropin Releasing Hormone (GnRH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gonadotropin Releasing Hormone (GnRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gonadotropin Releasing Hormone (GnRH) in the sample.

Alternative Names

GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1

Item Name

Gonadotropin Releasing Hormone

Research Area

Endocrinology;Reproductive science;Neuro science;Genetic science;Hormone metabolism;

Reference