ELISA Kit for Glycated Albumin (GA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

246.9-20,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 85.5ng/mL

Sample type

serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Glycated Albumin (GA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Glycated Albumin (GA) and unlabeled Glycated Albumin (GA) (Standards or samples) with the pre-coated antibody specific to Glycated Albumin (GA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glycated Albumin (GA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Glycated Albumin (GA) in the sample.

Alternative Names

Item Name

Glycated Albumin

Research Area

Reference