ELISA Kit for Gastrodin (GTD)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

246.9-20000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 87.5ng/mL

Sample type

Biological agents and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gastrodin (GTD) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gastrodin (GTD) and unlabeled Gastrodin (GTD) (Standards or samples) with the pre-coated antibody specific to Gastrodin (GTD). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gastrodin (GTD) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gastrodin (GTD) in the sample.

Alternative Names

Gastrodine

Item Name

Gastrodin

Research Area

Reference