ELISA Kit for Gamma-Carboxyglutamic Acid (gla)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

1.23-100ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.51ng/mL

Sample type

Biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gamma-Carboxyglutamic Acid (gla) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gamma-Carboxyglutamic Acid (gla) and unlabeled Gamma-Carboxyglutamic Acid (gla) (Standards or samples) with the pre-coated antibody specific to Gamma-Carboxyglutamic Acid (gla). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gamma-Carboxyglutamic Acid (gla) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gamma-Carboxyglutamic Acid (gla) in the sample.

Alternative Names

Gamma-Carboxyglutamate

Item Name

Gamma-Carboxyglutamic Acid

Research Area

Reference