ELISA Kit for Fibroblast Growth Factor 18 (FGF18)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

31.2-500pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 14.5pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 18 (FGF18) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 18 (FGF18) and unlabeled Fibroblast Growth Factor 18 (FGF18) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 18 (FGF18). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 18 (FGF18) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 18 (FGF18) in the sample.

Alternative Names

ZFGF5

Item Name

Fibroblast Growth Factor 18

Research Area

Cytokine;

Reference