ELISA Kit for Fibroblast Growth Factor 15 (FGF15)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.5-200pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.2pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 15 (FGF15) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 15 (FGF15) and unlabeled Fibroblast Growth Factor 15 (FGF15) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 15 (FGF15). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 15 (FGF15) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 15 (FGF15) in the sample.

Alternative Names

Item Name

Fibroblast Growth Factor 15

Research Area

Human gene deletion;

Reference

The relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of glucose intolerance;