ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

62.5-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Bos taurus; Bovine (Cattle)

Sensitivity

The minimum detectable dose of this kit is typically less than 26.6pg/mL.

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 1, Acidic (FGF1) and unlabeled Fibroblast Growth Factor 1, Acidic (FGF1) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 1, Acidic (FGF1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 1, Acidic (FGF1) in the sample.

Alternative Names

ECGF; AFGF; ECGFA; ECGFB; FGF-Alpha; FGFA; HBGF1; ECGFB; HBGF1; Acidic Fibroblast Growth Factor; heparin-binding growth factor 1; Endothelial Cell Growth Factor, Beta

Item Name

Fibroblast Growth Factor 1, Acidic

Research Area

Cytokine;Tumor immunity;Infection immunity;

Reference