ELISA Kit for Fibrinogen (FG)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

370.4-30,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 145.2ng/mL

Sample type

plasma

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibrinogen (FG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibrinogen (FG) and unlabeled Fibrinogen (FG) (Standards or samples) with the pre-coated antibody specific to Fibrinogen (FG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibrinogen (FG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibrinogen (FG) in the sample.

Alternative Names

F1; FI; Fbg; Coagulation Factor I

Item Name

Fibrinogen

Research Area

Reference