ELISA Kit for Fibrinogen Alpha Chain (FGA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

31.25-2000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 10.5ng/mL

Sample type

Plasma

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen Alpha Chain (FGA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibrinogen Alpha Chain (FGA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibrinogen Alpha Chain (FGA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibrinogen Alpha Chain (FGA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

Fib2; FG-A; FGA; Fibrinopeptide A

Item Name

Fibrinogen Alpha Chain

Research Area

Metabolic pathway;Hematology;

Reference