ELISA Kit for Ferroportin (FPN)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

246.9-20,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 91.4pg/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ferroportin (FPN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ferroportin (FPN) and unlabeled Ferroportin (FPN) (Standards or samples) with the pre-coated antibody specific to Ferroportin (FPN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ferroportin (FPN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ferroportin (FPN) in the sample.

Alternative Names

SLC40A1; FPN1; HFE4; IREG1; MTP1; SLC11A3; Solute Carrier Family 40,Member 1; Iron-Regulated Transporter

Item Name

Ferroportin

Research Area

Metabolic pathway;Infection immunity;Hematology;

Reference