ELISA Kit for Extracellular Signal Regulated Kinase 1 (ERK1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.625-40ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.258ng/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Extracellular Signal Regulated Kinase 1 (ERK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Extracellular Signal Regulated Kinase 1 (ERK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Extracellular Signal Regulated Kinase 1 (ERK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Extracellular Signal Regulated Kinase 1 (ERK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; MAPK3; ERT2; Mitogen-Activated Protein Kinase 3; Insulin-stimulated MAP2 kinase; Microtubule-associated protein 2 kinase

Item Name

Extracellular Signal Regulated Kinase 1

Research Area

Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;

Reference

Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome;