ELISA Kit for Endomorphin 2 (EM2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

3.70-300pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.56pg/mL

Sample type

Serum, plasma and other biological fluids.

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Endomorphin 2 (EM2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Endomorphin 2 (EM2) and unlabeled Endomorphin 2 (EM2) (Standards or samples) with the pre-coated antibody specific to Endomorphin 2 (EM2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Endomorphin 2 (EM2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Endomorphin 2 (EM2) in the sample.

Alternative Names

EP2

Item Name

Endomorphin 2

Research Area

Neuro science;Gastroenterology;Hormone metabolism;

Reference

An Engineered Endomorphin-2 Gene for Morphine Withdrawal Syndrome;