ELISA Kit for Elongation Of Very Long Chain Fatty Acids Like Protein 4 (ELOVL4)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.055ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elongation Of Very Long Chain Fatty Acids Like Protein 4 (ELOVL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elongation Of Very Long Chain Fatty Acids Like Protein 4 (ELOVL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elongation Of Very Long Chain Fatty Acids Like Protein 4 (ELOVL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elongation Of Very Long Chain Fatty Acids Like Protein 4 (ELOVL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

ADMD; STGD2; STGD3; CT118; Cancer/Testis Antigen 118; 3-keto acyl-CoA synthase ELOVL4; ELOVL fatty acid elongase 4; Very-long-chain 3-oxoacyl-CoA synthase 4

Item Name

Elongation Of Very Long Chain Fatty Acids Like Protein 4

Research Area

Metabolic pathway;

Reference