ELISA Kit for Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.055ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CD39; ATPDase; NTPDase-1; Ecto-ATP diphosphohydrolase 1; Ecto-apyrase; Lymphoid cell activation antigen

Item Name

Ectonucleoside Triphosphate Diphosphohydrolase 1

Research Area

CD & Adhesion molecule;Enzyme & Kinase;Metabolic pathway;Tumor immunity;

Reference

Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment …;Reduced Methylation Downregulates CD39/ENTPD1 and ZDHHC14 to Suppress Trophoblast Cell Proliferation and Invasion: Implications in Preeclampsia;