ELISA Kit for Dynamin 1 Like Protein (DNM1L)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.312-20ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.119ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynamin 1 Like Protein (DNM1L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dynamin 1 Like Protein (DNM1L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynamin 1 Like Protein (DNM1L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dynamin 1 Like Protein (DNM1L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

DRP1; DLP1; DVLP; DYMPLE; HDYNIV; VPS1; Dynamin-related protein 1; Dnm1p/Vps1p-like protein; Dynamin family member proline-rich carboxyl-terminal domain less

Item Name

Dynamin 1 Like Protein

Research Area

Neuro science;

Reference