ELISA Kit for Dihydropyrimidinase Like Protein 2 (DPYSL2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78-5000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 27pg/mL

Sample type

tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydropyrimidinase Like Protein 2 (DPYSL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydropyrimidinase Like Protein 2 (DPYSL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydropyrimidinase Like Protein 2 (DPYSL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydropyrimidinase Like Protein 2 (DPYSL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CRMP2; DHPRP2; DRP-2; DRP2; ULIP2; Unc-33-like phosphoprotein 2; Collapsin response mediator protein 2

Item Name

Dihydropyrimidinase Like Protein 2

Research Area

Neuro science;

Reference