ELISA Kit for Diacylglycerol (DAG)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

391-100,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 153pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Diacylglycerol (DAG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Diacylglycerol (DAG) and unlabeled Diacylglycerol (DAG) (Standards or samples) with the pre-coated antibody specific to Diacylglycerol (DAG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Diacylglycerol (DAG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Diacylglycerol (DAG) in the sample.

Alternative Names

DG; Diglyceride

Item Name

Diacylglycerol

Research Area

Signal transduction;Endocrinology;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism;

Reference

Wogonin ameliorates lipotoxicity-induced apoptosis of cultured vascular smooth muscle cells via interfering with DAG-PKC pathway;Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring rats;Aryl Hydrocarbon Receptor Plays Protective Roles against High Fat Diet (HFD)-induced Hepatic Steatosis and the Subsequent Lipotoxicity via Direct Transcriptional ReCavia (Guinea pig )lation of Socs3 Gene Expression;Pigment Epithelium-Derived Factor (PEDF) Improves Ischemic Cardiac Functional Reserve Through Decreasing Hypoxic Cardiomyocyte Contractility Through PEDF Receptor (PEDF-R).;PEDF protects cardiomyocytes by promoting FUNDC1‑mediated mitophagy via PEDF-R under hypoxic condition;Bouchardatine analogue alleviates NAFLD/NASH in high fat fed mice via blunting ATP synthase activity;