ELISA Kit for Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.057ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CYP21B; CPS1; CA21H; CAH1; CYP21; CYP21-B; 21-OHase; Cytochrome P450 21B; Steroid 21-hydroxylase; Cytochrome P450,Family 21,Subfamily A,Polypeptide 2

Item Name

Cytochrome P450 Family 21 Subfamily A Member 2

Research Area

Enzyme & Kinase;Metabolic pathway;Endocrinology;Hormone metabolism;

Reference