ELISA Kit for Cytochrome P450 1A2 (CYP1A2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.312-20ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.112ng/mL

Sample type

tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1A2 (CYP1A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 1A2 (CYP1A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 1A2 (CYP1A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 1A2 (CYP1A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CP12; P3-450; P450(PA); Cytochrome P450,Family 1,Subfamily A,Polypeptide 2

Item Name

Cytochrome P450 1A2

Research Area

Metabolic pathway;

Reference

Liver lobe and strain differences in the activity of murine cytochrome p450 enzymes;Stammesspezifische Unterschiede in der analgetischen Wirkung von Buprenorphin bei der Maus;