ELISA Kit for Cross Linked C-Telopeptide Of Type III Collagen (CTXIII)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.5-10,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 43.3pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cross Linked C-Telopeptide Of Type III Collagen (CTXIII) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cross Linked C-Telopeptide Of Type III Collagen (CTXIII) and unlabeled Cross Linked C-Telopeptide Of Type III Collagen (CTXIII) (Standards or samples) with the pre-coated antibody specific to Cross Linked C-Telopeptide Of Type III Collagen (CTXIII). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type III Collagen (CTXIII) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type III Collagen (CTXIII) in the sample.

Alternative Names

CTX-III; CTX3

Item Name

Cross Linked C-Telopeptide Of Type III Collagen

Research Area

Metabolic pathway;Nutrition metabolism;Bone metabolism;

Reference