ELISA Kit for Creatinine (CTN)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

2.47-200ug/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.97ug/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Creatinine (CTN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Creatinine (CTN) and unlabeled Creatinine (CTN) (Standards or samples) with the pre-coated antibody specific to Creatinine (CTN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Creatinine (CTN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Creatinine (CTN) in the sample.

Alternative Names

Cr

Item Name

Creatinine

Research Area

Reference