ELISA Kit for Creatine Transporter (CRT)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

24.69-2,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 8.55pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Creatine Transporter (CRT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Creatine Transporter (CRT) and unlabeled Creatine Transporter (CRT) (Standards or samples) with the pre-coated antibody specific to Creatine Transporter (CRT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Creatine Transporter (CRT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Creatine Transporter (CRT) in the sample.

Alternative Names

SLC6A8; CT1; CRTR; Sodium And Chloride Dependent Creatine Transporter 1; Solute Carrier Family 6 Member 8

Item Name

Creatine Transporter

Research Area

Signal transduction;

Reference