ELISA Kit for Collagen Type I (COL1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

18.52-1,500ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Equus caballus; Equine (Horse)

Sensitivity

The minimum detectable dose of this kit is typically less than 7.15ng/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Collagen Type I (COL1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Collagen Type I (COL1) and unlabeled Collagen Type I (COL1) (Standards or samples) with the pre-coated antibody specific to Collagen Type I (COL1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Collagen Type I (COL1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Collagen Type I (COL1) in the sample.

Alternative Names

Item Name

Collagen Type I

Research Area

Metabolic pathway;Developmental science;Bone metabolism;

Reference