ELISA Kit for Coagulation Factor XIII B Polypeptide (F13B)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

617.3-50,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 243.7ng/mL

Sample type

plasma

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Coagulation Factor XIII B Polypeptide (F13B) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Coagulation Factor XIII B Polypeptide (F13B) and unlabeled Coagulation Factor XIII B Polypeptide (F13B) (Standards or samples) with the pre-coated antibody specific to Coagulation Factor XIII B Polypeptide (F13B). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Coagulation Factor XIII B Polypeptide (F13B) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Coagulation Factor XIII B Polypeptide (F13B) in the sample.

Alternative Names

F13-B; FXIIIB; Fibrin-stabilizing factor B subunit; Protein-glutamine gamma-glutamyltransferase B chain; Transglutaminase B chain

Item Name

Coagulation Factor XIII B Polypeptide

Research Area

Signal transduction;Metabolic pathway;Hematology;

Reference