ELISA Kit for Cluster Of Differentiation 30 Ligand (CD30L)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

125-8000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 50pg/mL

Sample type

serum, plasma, tissue homogenates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 30 Ligand (CD30L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 30 Ligand (CD30L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 30 Ligand (CD30L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 30 Ligand (CD30L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CD153; TNFSF8; CD30-L; Tumor Necrosis Factor Ligand Superfamily Member 8

Item Name

Cluster Of Differentiation 30 Ligand

Research Area

Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;

Reference