ELISA Kit for Cinobufagin (CBG)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

246.9-20,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 91.3ng/mL

Sample type

Biological agents and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cinobufagin (CBG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cinobufagin (CBG) and unlabeled Cinobufagin (CBG) (Standards or samples) with the pre-coated antibody specific to Cinobufagin (CBG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cinobufagin (CBG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cinobufagin (CBG) in the sample.

Alternative Names

Item Name

Cinobufagin

Research Area

Reference