ELISA Kit for Chromogranin A (CHGA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

125-8,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 48pg/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromogranin A (CHGA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chromogranin A (CHGA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chromogranin A (CHGA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chromogranin A (CHGA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CGA; SP-I; Parathyroid Secretory Protein 1; Vasostatin; Pancreastatin; Parastatin; Pituitary secretory protein I

Item Name

Chromogranin A

Research Area

Tumor immunity;Neuro science;

Reference