ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

3.12-50ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.38ng/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chorionic Gonadotropin Beta Polypeptide (CGb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chorionic Gonadotropin Beta Polypeptide (CGb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CG-B; CGB3; hCGB; HCG; CGb5; CGb7; CGb8; Choriogonadotropin subunit beta

Item Name

Chorionic Gonadotropin Beta Polypeptide

Research Area

Reproductive science;

Reference

联合检测血清血管内皮生长因子和β-HCG对早期异位妊娠的诊断意义;Regulation of circulating chromogranin B levels in heart failure;