ELISA Kit for Chorionic Gonadotropin Alpha Polypeptide (CGa)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.5-10,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 49.4pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Chorionic Gonadotropin Alpha Polypeptide (CGa) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Chorionic Gonadotropin Alpha Polypeptide (CGa) and unlabeled Chorionic Gonadotropin Alpha Polypeptide (CGa) (Standards or samples) with the pre-coated antibody specific to Chorionic Gonadotropin Alpha Polypeptide (CGa). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Chorionic Gonadotropin Alpha Polypeptide (CGa) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Chorionic Gonadotropin Alpha Polypeptide (CGa) in the sample.

Alternative Names

FSHA; GPHA1; GPHa; HCG; LHA; TSHA; Thyroid Stimulating Hormone Alpha; Follicle-Stimulating Hormone Alpha Subunit; Luteinizing hormone alpha chain; Lutropin alpha chain

Item Name

Chorionic Gonadotropin Alpha Polypeptide

Research Area

Tumor immunity;Endocrinology;Reproductive science;Genetic science;Hormone metabolism;

Reference