ELISA Kit for Cholecystokinin 18 (CCK18)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.39pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cholecystokinin 18 (CCK18) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cholecystokinin 18 (CCK18) and unlabeled Cholecystokinin 18 (CCK18) (Standards or samples) with the pre-coated antibody specific to Cholecystokinin 18 (CCK18). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cholecystokinin 18 (CCK18) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cholecystokinin 18 (CCK18) in the sample.

Alternative Names

Item Name

Cholecystokinin 18

Research Area

Reference