ELISA Kit for Chemokine (C-X-C motif) ligand 7 ( CXCL7)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.24-15ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Oryctolagus cuniculus (Rabbit)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.11ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine (C-X-C motif) ligand 7 ( CXCL7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine (C-X-C motif) ligand 7 ( CXCL7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine (C-X-C motif) ligand 7 ( CXCL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine (C-X-C motif) ligand 7 ( CXCL7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CXCL7; PPBP; PBP; B-TG1; CTAP-III; CTAP3; CTAPIII; LA-PF4; LDGF; MDGF; NAP2; SCYB7; TC1; TC2; TGB1; THBGB1; Pro-Platelet Basic Protein; Beta-Thromboglobulin

Item Name

Chemokine (C-X-C motif) ligand 7

Research Area

Cytokine;Hematology;

Reference