ELISA Kit for Chemokine (C-X-C Motif) Ligand 2 (CXCL2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

15.6-1000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.7pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine (C-X-C Motif) Ligand 2 (CXCL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine (C-X-C Motif) Ligand 2 (CXCL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine (C-X-C Motif) Ligand 2 (CXCL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine (C-X-C Motif) Ligand 2 (CXCL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

GROb; SCYB2; MIP2; GRO2; MIP2a; MGSAb; CINC2a; HSF; Macrophage inflammatory protein 2-alpha; Hematopoietic synergistic factor; Growth Regulated Oncogene Beta

Item Name

Chemokine (C-X-C Motif) Ligand 2

Research Area

Cytokine;Tumor immunity;Infection immunity;

Reference

HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis;Protein Inhibitor of Activated STAT 1 (PIAS1) Protects Against Obesity-Induced Insulin Resistance by Inhibiting Inflammation Cascade in Adipose Tissue;Targeting peptidylarginine deiminase reduces neutrophil extracellular trap formation and tissue injury in severe acute pancreatitis;Integrated Omics Reveals Tollip as an Aggravator and Therapeutic Target for Hepatic Ischemia‐Reperfusion Injury in Mice;