ELISA Kit for Chemokine C-C-Motif Receptor 7 (CCR7)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.055ng/mL

Sample type

tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Receptor 7 (CCR7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Receptor 7 (CCR7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Receptor 7 (CCR7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Receptor 7 (CCR7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CD197; CCR7; BLR2; CDw197; CMKBR7; EBI1; Epstein-Barr Virus Induced Protein 1; EBV-induced G-protein coupled receptor 1; MIP-3 beta receptor

Item Name

Chemokine C-C-Motif Receptor 7

Research Area

Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;

Reference