ELISA Kit for Chemokine C-C-Motif Ligand 16 (CCL16)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.066ng/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Ligand 16 (CCL16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Ligand 16 (CCL16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Ligand 16 (CCL16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Ligand 16 (CCL16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

SCYA16; SCYL4; LEC; HCC4; LMC; LCC1; CKb12; Mtn1; CC-4; Monotactin-1; IL-10-inducible chemokine; Liver-expressed chemokine; Lymphocyte and monocyte chemoattractant

Item Name

Chemokine C-C-Motif Ligand 16

Research Area

Cytokine;Tumor immunity;Infection immunity;Hepatology;

Reference

Soluble CXCL16 Promotes TNF‐α‐induced Apoptosis in DLBCL via the AMAD10‐NF‐κB Regulatory Feedback Loop;