ELISA Kit for Cathelicidin Antimicrobial Peptide (CAMP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.5-10,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 49.2ng/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cathelicidin Antimicrobial Peptide (CAMP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cathelicidin Antimicrobial Peptide (CAMP) and unlabeled Cathelicidin Antimicrobial Peptide (CAMP) (Standards or samples) with the pre-coated antibody specific to Cathelicidin Antimicrobial Peptide (CAMP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cathelicidin Antimicrobial Peptide (CAMP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cathelicidin Antimicrobial Peptide (CAMP) in the sample.

Alternative Names

CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic

Item Name

Cathelicidin Antimicrobial Peptide

Research Area

Infection immunity;

Reference

The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis;Host immune response to tuberculous meningitis;On birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experiment;Modulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells;Cathelicidin as a link between sarcoidosis and tuberculosis;The association of vitamin D, cathelicidin, and vitamin D binding protein with acute asthma attacks in children;Swiftly Decreasing Cerebrospinal Fluid Cathelicidin Concentration Predicts Improved Outcome in Childhood Bacterial Meningitis.;