ELISA Kit for Carnosine (Car)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

11.11-900ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.51ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Carnosine (Car) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Carnosine (Car) and unlabeled Carnosine (Car) (Standards or samples) with the pre-coated antibody specific to Carnosine (Car). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Carnosine (Car) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Carnosine (Car) in the sample.

Alternative Names

β-Alanyl-L-Histidine

Item Name

Carnosine

Research Area

Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;

Reference

Dietary supplemental vitamin B6 increases carnosine and anserine concentrations in the heart of rats;Zinc supplementation influences genomic stability biomarkers, antioxidant activity, and zinc transporter genes in an elderly Australian population with low zinc status;The effect of 12 weeks carnosine supplementation on renal functional integrity and oxidative stress in pediatric patients with diabetic nephropathy: a randomized placebo-controlled trial;