ELISA Kit for Calbindin (CALB)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.067ng/mL

Sample type

tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calbindin (CALB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calbindin (CALB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calbindin (CALB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calbindin (CALB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CAB27; Calbindin 1,28kDa; Calbindin D28; Vitamin D-dependent calcium-binding protein, avian-type

Item Name

Calbindin

Research Area

Metabolic pathway;

Reference

敲除小鼠足细胞核因子-κB 受体活化因子基因对尿蛋白的影响;