ELISA Kit for C-Type Natriuretic Peptide (CNP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than

Sample type

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to C-Type Natriuretic Peptide (CNP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled C-Type Natriuretic Peptide (CNP) and unlabeled C-Type Natriuretic Peptide (CNP) (Standards or samples) with the pre-coated antibody specific to C-Type Natriuretic Peptide (CNP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of C-Type Natriuretic Peptide (CNP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of C-Type Natriuretic Peptide (CNP) in the sample.

Alternative Names

Item Name

C-Type Natriuretic Peptide

Research Area

Cardiovascular biology;Hormone metabolism;

Reference